Summary
Type: SPE
Application #: 301440
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
1 column volume n-hexane, then 1 column volume methanol
Sample application:
Force or aspirate 0.5 ml of the clear supernatant through the column
Column washing:
500 μl acetonitrile / water (1 : 1, v/v); dry column under vacuum for 10 min
Elution:
3 x 500 μl n-hexane; concentrate eluate in a stream of nitrogen and redissolve
in 100 μl n-hexane
Authors: LC Department
Source: Macherey-Nagel